Journal: Nature protocols
Article Title: An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity.
doi: 10.1038/s41596-020-0401-x
Figure Lengend Snippet: Fig. 9 | Examples of results obtained with a BER assay. Results were produced using substrate cells (HeLa) exposed to Ro 19-8022 + light (gray bars) or non-exposed substrate cells (white bars) incubated with various protein extracts from piglet hippocampus samples (n = 5, Sus scrofa domesticus)31. a,b, Example of a successful experiment (a) and example of a suboptimal experiment with high background values (b). Data are shown as mean percentage of tail DNA for two independent incubations with standard deviation within one experiment.
Article Snippet: ● Formamidopyrimidine DNA glycosylase (Fpg) as incubation reaction control for BER (New England BioLabs, cat. no. M0240S; Norgenotech, cat. no. E0103-10) ● Human 8-oxo-guanine DNA glycosylase (hOGG1) as alternative incubation reaction control for BER (Trevigen, cat. no. 4130-100-EB) ● T4 Endonuclease V (T4endoV) – as incubation reaction control for NER (New England BioLabs, cat. no. M0308S)
Techniques: Produced, Incubation, Standard Deviation